Kidney Research: Experimental Protocols / Edition 1

by Tim D. Hewitson, Gavin J. Becker

Overview

As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment. The study of renal disease, however, suffers due to the complex nature of renal anatomy and physiology and the plethora of different cell types found in the kidney. In Kidney Research: Experimental Prools, top experts in the field seek to aid researchers by providing a number of specialized techniques developed to examine this intricate system. Through both well-established and novel methods, this volume explores the preparation and culture of the main cell types used to study renal disease mechanisms, the common animal models used to mimic the various forms of human renal disease, and specific applications and techniques used in vivo and in vitro. Written in the highly successful Methods in Molecular Biology™ series format, the chapters contain step-by-step, readily reproducible laboratory prools, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.

Essential and cutting-edge, Kidney Research: Experimental Prools delivers invaluable guidance to new and experienced laboratory researchers delving into the sophisticated study of the kidney.

Product Details

ISBN-13:	9781588299451
Publisher:	Springer-Verlag New York, LLC
Publication date:	12/04/2008
Series:	Methods in Molecular Biology , #466
Edition description:	2009
Pages:	276
Product dimensions:	6.30(w) x 9.30(h) x 0.80(d)

Table of Contents

Isolation and Propagation of Cell Populations.- Isolation and Propagation of Glomerular Mesangial Cells.- Isolation and Primary Culture of Human Proximal Tubule Cells.- Propagation and Culture of Renal Fibroblasts.- Animal Models.- Small Animal Models of Kidney Disease: A Review.- Techniques and Applications.- Measurement of Glomerular Filtration Rate in Conscious Mice.- Laser Capture Microdissection of Kidney Tissue.- Quantitative Gene Expression Analysis in Kidney Tissues.- In Vivo Imaging of Leukocyte Recruitment to Glomeruli in Mice Using Intravital Microscopy.- Using In Situ Hybridisation to Localize Renal Gene Expression in Tissue Sections.- Immuno and Lectin Hishemistry for Renal Light Microscopy.- Immuno and Lectin Hishemistry for Renal Electron Microscopy.- Pimonidazole Adduct Immunohishemistry in the Rat Kidney: Detection of Tissue Hypoxia.- Identification of Apoptosis in Kidney Tissue Sections.- Cell-Populated Floating Collagen Lattices: An In Vitro Model of Parenchymal Contraction.- Mechanical Stretch-Induced Signal Transduction in Cultured Mesangial Cells.- Determination of Collagen Content, Concentration, and Sub-types in Kidney Tissue.- SELDI-TOF Mass Spectrometry-Based Protein Profiling of Kidney Tissue.- In Vivo Transfer of Small Interfering RNA or Small Hairpin RNA Targeting Glomeruli.

Editorial Reviews

Reviewer: Jens Goebel, MD (Cincinnati Children's Hospital Medical Center)
Description: This book details research methodologies especially relevant for nephrological studies.
Purpose: The purpose is to collect and present research protocols appropriate for nephrological investigations. This is a worthy objective that the book by and large meets.
Audience: Researchers in the field of nephrology are the intended audience.
Features: The book covers research methodologies used in renal studies (as well as in other studies), including proteomics, micro-RNA work, and other cutting-edge approaches. The major weakness of the book is the at times insufficient quality of the figures (e.g. Fig. 10.3.a. where not much specific information can be discerned without arrows or other visual aids).
Assessment: This is a unique book that has no competitors. For those of us who still enjoy and rely on books as references, it is clearly of some value.

Doody's Review Service

From the reviews:

“This book details research methodologies especially relevant for nephrological studies. … Researchers in the field of nephrology are the intended audience. The book covers research methodologies used in renal studies (as well as in other studies), including proteomics, micro-RNA work, and other cutting-edge approaches. … This is a unique book that has no competitors.” (Jens Goebel, Doody’s Book Reviews, December, 2011)

From the Publisher