Next-Generation Sequencing: Current Technologies and Applications

by Jianping Xu (Editor)

Overview

High-throughput, next generation sequencing (NGS) technologies are capable of producing a huge amount of sequence data in a relatively short time and have revolutionized genome research in recent years. The powerful and flexible nature of NGS has made it an indispensable tool for a broad spectrum of biological sciences, and NGS technologies have transformed scientific research in many fields. Written by experts from around the world, this book explores the most recent advances in NGS instrumentation and data analysis. The book begins with a comprehensive description of current NGS platforms, their sequencing chemistries, instrument specifications, and general workflows and procedures. A separate chapter is dedicated to low-quantity, single molecule sequencing technology. Further chapters explore the application of NGS technologies in various fields, including polymorphism detection, sRNA research, rare variant detection, large variant detection, exome sequencing, plant development studies, microbial metagenomics, and studies on the human microbiome. Practical and cutting-edge, this volume will assist all scientists who wish to apply these innovative research tools.

Product Details

ISBN-13:	9781908230331
Publisher:	Caister Academic Press Limited
Publication date:	02/01/2014
Pages:	172
Product dimensions:	7.00(w) x 9.90(h) x 0.60(d)

Table of Contents

Contributors v

Preface ix

1 An Overview of Next-generation Genome Sequencing Platforms Chandra Shekhar Pareek 1

2 Attomole-level Genomics with Single-molecule Direct DNA, cDNA and RNA Sequencing Technologies Fatih Ozsolak 25

3 SNP Assessment on Draft Genomes from Next-generation Sequencing Data Michael Piechotta Christoph Dieterich 33

4 Processing Large-scale Small RNA Datasets in Silico Daniel Mapleson Irina Mohorianu Helio Pais Matthew Stocks Leighton Folkes Vincent Moulton 41

5 Utility of High-throughput Sequence Data in Rare Variant Detection Viacheslav Y. Fofanov Tudor Constantin Heather Koshinsky 67

6 Detecting Breakpoints of Insertions and Deletions from Paired-end Short Reads Kai Ye Zemin Ning 83

7 Novel Insights from Re-sequencing of Human Exomes Through NGS Tao Jiang Jun Li Xu Yang Jun Wang 91

8 Insights on Plant Development Using NGS Technologies Ying Wang Yuling Jiao 121

9 Next-generation Sequencing and the Future of Microbial Metagenomics Andreas Wilke Peter Larsen Jack A. Gilbert 131

10 Next-generation Sequencing, Metagenomes and the Human Microbiome Karen E. Nelson Ramana Madupu Sebastian Szpakowski Johannes B. Goll Konstantinos Krampis Barbara A. Methé 141

Index 155